Structural studies on sterol carrier protein-2 from plants

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Primary sequence and structural analysis of sterol carrier protein 2 from rat liver: homology with immunoglobulins.

Sterol carrier protein 2 (SCP2) is involved in the later steps of cholesterol biosynthesis and in the intracellular transport of cholesterol. In the present investigation, the amino acid sequence of SCP2 from rat liver has been determined. It is a single polypeptide chain with 122 amino acid residues. Secondary structure prediction indicates an amphipathic alpha-helix region for residues 21-34 ...

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Studies on the Synthesis of Sterol Carrier Protein-2 in Rat Adrenocortical Cells in Monolayer Culture

The effects of ACTH or dibutyryl cyclic AMP (Bt2 CAMP) on the synthesis of sterol carrier protein-2 (SCP2) have been studied in rat adrenocortical cells in monolayer culture. Radiolabeling of total cellular proteins with [3SS]methionine and immunoprecipitation with antibodies directed against rat liver SCP2, followed by polyacrylamide gel electrophoresis and fluorography, showed a 3-4-fold incr...

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Sterol carrier protein-2/sterol carrier protein-x expression differentially alters fatty acid metabolism in L cell fibroblasts.

Sterol carrier protein-2 (SCP-2) and SCP-x are ubiquitous proteins found in all mammalian tissues. Although both proteins interact with fatty acids, their relative contributions to the uptake, oxidation, and esterification of straight-chain (palmitic) and branched-chain (phytanic) fatty acids in living cells has not been resolved. Therefore, the effects of each gene product on fatty acid metabo...

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Participation of sterol carrier protein-2 in cholesterol metabolism.

818 Introduction Lipid transport between intracellular organelles is thought to involve carrier proteins. Several different lipid transfer proteins have been isolated which are involved in the transport of phosphatidylcholine, phosphatidylinositol, phosphatidylserine, cholesterol, gangliosides, glycolipids, dolichol and vitamin D3 [ 1-31. In addition to protein mediated transport, other process...

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In Vivo Functional Genomic Studies of Sterol Carrier Protein-2 Gene in the Yellow Fever Mosquito

A simple and efficient DNA delivery method to introduce extrachromosomal DNA into mosquito embryos would significantly aid functional genomic studies. The conventional method for delivery of DNA into insects is to inject the DNA directly into the embryos. Taking advantage of the unique aspects of mosquito reproductive physiology during vitellogenesis and an in vivo transfection reagent that med...

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ژورنال

عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography

سال: 2006

ISSN: 0108-7673

DOI: 10.1107/s0108767306097170